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Baird-Parker Medium (BAM M17)

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

Baird-Parker Medium

(BAM M17)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

Glycine..............................................................12.0g

Sodium pyruvate...............................................10.0g

Pancreatic digest of casein................................10.0g

Beef extract.........................................................5.0g

LiCl·6H2O...........................................................5.0g

Yeast extract........................................................1.0g

EY tellurite enrichment................................. 50.0mL

pH 7.0 ± 0.2 at 25°C

EY Tellurite Enrichment:

Composition per 100.0mL:

Chicken egg yolks..................................................10

K2TeO3 ..............................................................0.15g

NaCl (0.9% solution) .................................... 50.0mL

Preparation of EY Tellurite Enrichment:

Soak eggs with 1:100 dilution of saturated mercuric

chloride solution for 1 min. Crack 11 eggs and sepa

rate yolks from whites. Mix egg yolks. Measure

30.0mL of egg yolk emulsion and add to 70.0mL of

0.9% NaCl solution. Mix thoroughly. Add 0.15g

K2TeO3. Filter sterilize. Warm to 45°–50°C.

Caution: Potassium tellurite is toxic.

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept EY tellurite enrichment, to distilled/deionized

water and bring volume to 950.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 48°–50°C.

Aseptically add 50.0mL of sterile EY tellurite enrich

ment. Mix thoroughly. Pour into sterile Petri dishes.

The medium must be densely opaque. Dry plates be

fore use. Plates can be stored for up to 5 days at 20–

25°C before use.

Use: For the selective isolation and enumeration of co

agulase-positive staphylococci from foods.