培养基配方

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Basal Synthetic Medium

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

L-Glutamic acid.................................................20.0g

(NH4)2SO4 ..........................................................4.0g

K2HPO4.............................................................1.88g

KH2PO4.............................................................0.57g

MgSO4·7H2O......................................................0.2g

Salt solution .................................................. 10.0mL

Salt Solution:

Composition per liter:

FeCl3·6H2O.........................................................0.6g

MnCl2·4H2O .......................................................0.6g

ZnCl2...................................................................0.6g

CuSO4·5H2O.......................................................0.6g

CaCl2·2H2O ........................................................0.6g

NaCl....................................................................0.6g

Preparation of Salt Solution: Add components

to 1.0L of distilled/deionized water. Mix thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Acine

tobacter lwoffii.