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BCP Azide Broth (Bromcresol Purple Azide Broth)

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

BCP Azide Broth

(Bromcresol Purple Azide Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Casein peptone..................................................10.0g

Yeast extract......................................................10.0g

D-Glucose............................................................5.0g

NaCl....................................................................5.0g

K2HPO4...............................................................2.7g

KH2PO4...............................................................2.7g

NaN3 ...................................................................0.5g

Bromcresol Purple ..........................................0.032g

pH 6.9 ± 0.2 at 25°C

Caution: Sodium azide is toxic. Azides also react

with metals and disposal must be highly diluted.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat to boiling. Distribute

into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C.

Use: For use in the confirmation test for the presence

of fecal streptococci.