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BCP D Agar (Bromcresol Purple Deoxycholate Agar)

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

BCP D Agar

(Bromcresol Purple Deoxycholate Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................25.0g

Lactose..............................................................10.0g

Sucrose..............................................................10.0g

Pancreatic digest of casein..................................7.5g

Thiopeptone ........................................................7.5g

NaCl....................................................................5.0g

Yeast extract........................................................2.0g

Sodium citrate.....................................................2.0g

Sodium deoxycholate..........................................1.0g

Bromcresol Purple ............................................0.02g

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Pour into sterile Petri dishes without sterilization. Do

not autoclave. Use the same day.

Use: For the isolation, cultivation, and differentiation

of Gram-negative enteric bacilli. For the isolation and

cultivation of Salmonella, Shigella, and other nonlac

tose- and nonsucrose-fermenting microorganisms.

Nonlactose/nonsucrose fermenting microorganisms

appear as colorless or blue colonies. Lactose/sucrose

fermenting microorganisms, such as coliform bacteria,

appear as yellow-opaque white colonies surrounded

by a zone of precipitated deoxycholate.