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BCP DCLS Agar (Bromcresol Purple Deoxycholate Citrate Lactose Sucrose Agar)

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

BCP DCLS Agar

(Bromcresol Purple Deoxycholate

Citrate Lactose Sucrose Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................14.0g

Sodium citrate...................................................10.0g

Lactose................................................................7.5g

Sucrose................................................................7.5g

Pancreatic digest of casein..................................7.5g

Peptone................................................................7.5g

NaCl....................................................................5.0g

Na2S2O3·5H2O ....................................................5.0g

Yeast extract........................................................3.0g

Meat extract ........................................................3.0g

Sodium deoxycholate..........................................2.5g

Bromcresol Purple ............................................0.02g

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Pour into sterile Petri dishes without sterilization. Do

not autoclave. Use the same day.

Use: For the differential isolation of Gram-negative en

teric bacilli. For the isolation of Salmonella, Shigella,

and other nonlactose- and nonsucrose-fermenting mi

croorganisms. Nonlactose/nonsucrose-fermenting mi

croorganisms appear as colorless or blue colonies.

Lactose/sucrose-fermenting microorganisms, such as

coliform bacteria, appear as yellow-opaque white colo

nies surrounded by a zone of precipitated deoxycholate.