培养基配方

您现在的位置：网站首页 >> 培养基配方

Bile Esculin Agar

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

Bile Esculin Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Oxgall ...............................................................20.0g

Agar ..................................................................15.0g

Pancreatic digest of gelatin.................................5.0g

Beef extract.........................................................3.0g

Esculin ................................................................1.0g

Ferric citrate........................................................0.5g

Horse serum.................................................. 50.0mL

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid and BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept horse serum, to distilled/deionized water and

bring volume to 950.0L. Mix thoroughly and heat

with frequent agitation until boiling. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 50.0mL of filter sterilized horse se

rum. Distribute into sterile Petri dishes or test tubes.

Cool tubes in a slanted position.

Use: For differentiation between group D strepto

cocci and nongroup D streptococci. To differentiate

members of the Enterobacteriaceae, particularly

Klebsiella, Enterobacter, and Serratia, from other

enteric bacteria. To differentiate Listeria monocyto

genes. Bile tolerance and esculin hydrolysis (seen as

a dark brown to black complex) are presumptive for

enterococci (group D streptococci).