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Bile Esculin Agar (BAM M18)

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

Bile Esculin Agar

(BAM M18)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Oxgall ...............................................................40.0g

Agar ..................................................................15.0g

Pancreatic digest of gelatin.................................5.0g

Beef extract.........................................................3.0g

Esculin ................................................................1.0g

Ferric citrate........................................................0.5g

pH 6.6 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly and heat with frequent agitation until

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Distribute into sterile Petri dishes or test

tubes. Cool tubes in a slanted position.

Use: For differentiation between group D strepto

cocci and nongroup D streptococci. To differentiate

members of the Enterobacteriaceae, particularly

Klebsiella, Enterobacter, and Serratia, from other

enteric bacteria. To differentiate Listeria monocyto

genes. Bile tolerance and esculin hydrolysis (seen as

a dark brown to black complex) are presumptive for

enterococci (group D streptococci).