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Bile Oxalate Sorbose Broth (BOS Broth)

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

Bile Oxalate Sorbose Broth

(BOS Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2HPO4 ...........................................................9.14g

Sodium oxalate ...................................................5.0g

Bile salts..............................................................2.0g

NaCl....................................................................1.0g

CaCl2·2H2O.......................................................0.01g

MgSO4·7H2O ....................................................0.01g

Asparagine solution .................................... 100.0mL

Methionine solution .................................... 100.0mL

Sorbose solution.......................................... 100.0mL

Yeast extract solution.................................... 10.0mL

Sodium pyruvate solution ............................. 10.0mL

Metanil Yellow solution................................ 10.0mL

Sodium nitrofurantoin solution..................... 10.0mL

Irgasan® solution............................................ 1.0mL

pH 7.6 ± 0.2 at 25°C

Asparagine Solution:

Composition per 100.0mL:

Asparagine ..........................................................1.0g

Preparation of Asparagine Solution: Add as

paragine to distilled/deionized water and bring vol

ume to 100.0mL. Mix thoroughly. Filter sterilize.

Methionine Solution:

Composition per 100.0mL:

Methionine..........................................................1.0g

Preparation of Methionine Solution: Add me

thionine to distilled/deionized water and bring vol

ume to 100.0mL. Mix thoroughly. Filter sterilize.

Sorbose Solution:

Composition per 100.0mL:

Sorbose..............................................................10.0g

Preparation of Sorbose Solution: Add sorbose

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Yeast Extract Solution:

Composition per 10.0mL:

Yeast extract....................................................0.025g

Preparation of Yeast Extract Solution: Add

yeast extract to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Sodium Pyruvate Solution:

Composition per 10.0mL:

Sodium pyruvate...............................................0.05g

Preparation of Sodium Pyruvate Solution:

Add sodium pyruvate to distilled/deionized water

and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Metanil Yellow Solution:

Composition per 10.0mL:

Metanil Yellow ...............................................0.025g

Preparation of Metanil Yellow Solution: Add

Metanil Yellow to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Sodium Nitrofurantoin Solution:

Composition per 10.0mL:

Sodium nitrofurantoin.......................................0.01g

Preparation of Sodium Nitrofurantoin Solu

tion: Add sodium nitrofurantoin to distilled/deion

ized water and bring volume to 10.0mL. Mix

thoroughly. Filter sterilize.

Irgasan Solution:

Composition per 10.0mL:

Irgasan...............................................................0.04g

Ethanol (95% solution)................................. 10.0mL

Preparation of Irgasan Solution: Add Irgasan to

10.0mL of ethanol. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept asparagine solution, methionine solution, sorbose

solution, yeast extract solution, sodium pyruvate solu

tion, Metanil Yellow solution, sodium nitrofurantoin

solution, and Irgasan solution, to distilled/deionized

water and bring volume to 659.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti

cally add 100.0mL of sterile asparagine solution,

100.0mL of sterile methionine solution, 100.0mL of

sterile sorbose solution, 10.0mL of sterile yeast extract

solution, 10.0mL of sterile sodium pyruvate solution,

10.0mL of sterile Metanil Yellow solution, 10.0mL of

sterile sodium nitrofurantoin solution, and 1.0mL of

sterile Irgasan solution. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Yersinia en

terocolitica from foods.

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Bile Oxalate Sorbose Broth (BOS Broth)