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Bismuth Sulfite Broth (m-Bismuth Sulfite Broth)

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

Bismuth Sulfite Broth

(m-Bismuth Sulfite Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Bi2(SO3)3...........................................................16.0g

Pancreatic digest of casein................................10.0g

Peptic digest of animal tissue............................10.0g

Beef extract.......................................................10.0g

Glucose .............................................................10.0g

Na2HPO4 .............................................................8.0g

FeSO4·7H2O........................................................0.6g

pH 7.7 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly and heat with frequent agitation until

boiling. Boil for 1 min. Do not autoclave. Cool to

45°–50°C. Mix to disperse the precipitate and asepti

cally distribute into sterile tubes or flasks. Use 2.0–

2.2mL of medium for each membrane filter.

Use: For the selective isolation of Salmonella typhi

and other enteric bacilli and for the detection of Sal

monella by the membrane filter method.