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BL Agar (Glucose Blood Liver Agar)

[所属分类:培养基配方] [发布时间:2021-11-3] [发布人:网站管理员2] [阅读次数:] [返回]

BL Agar

(Glucose Blood Liver Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Glucose .............................................................10.0g

Proteose peptone No. 3.....................................10.0g

Pancreatic digest of casein..................................5.0g

Yeast extract........................................................5.0g

Meat extract ........................................................3.0g

Phytone™ ...........................................................3.0g

Tween 80.............................................................1.0g

Soluble starch......................................................0.5g

Liver extract................................................ 150.0mL

Horse blood................................................... 50.0mL

L-Cysteine·HCl solution ............................... 10.0mL

Solution A..................................................... 10.0mL

Solution B ....................................................... 5.0mL

pH 7.2 ± 0.2 at 25°C

Liver Extract:

Composition per 170.0mL:

Liver powder.....................................................10.0g

Preparation of Liver Extract: Add 10.0g of liv

er powder to 170mL of distilled/deionized water.

Gently heat to 60°C. Maintain at 50°–60°C for 1 hr.

Gently bring to boiling. Boil for 5 min. Adjust pH to

7.2. Filter through Whatman #2 filter paper.

L-Cysteine·HCl Solution:

Composition per 10.0mL:

L-Cysteine·HCl ...................................................0.5g

Preparation of L-Cysteine·HCl Solution: Dis

solve 0.5g of L-cysteine·HCl in distilled/deionized

water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize. Warm to 50°C.

Solution A:

Composition per 100.0mL:

K2HPO4.............................................................10.0g

KH2PO4.............................................................10.0g

Preparation of Solution A: Add components to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°–55°C.

Solution B:

Composition per 100.0mL:

MgSO4·7H2O......................................................4.0g

NaCl....................................................................0.2g

FeSO4·7H2O........................................................0.2g

MnSO4·H2O........................................................0.2g

Preparation of Solution B: Add components to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°–55°C.

Preparation of Medium: Add components, ex

cept liver extract, horse blood, L-cysteine·HCl solu

tion, solution A, and solution B, to distilled/

deionized water and bring volume to 775.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

50°–55°C. Aseptically add 150.0mL of sterile liver

extract, 50.0mL of sterile horse blood, 10.0mL of

sterile L-cysteine·HCl solution, 10.0mL of sterile so

lution A, and 5.0mL of sterile solution B. Mix thor

oughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the cultivation and maintenance of

Clostridium species, numerous Lactobacillus spe

cies, Lactococcus lactis, Leuconostoc lactis, Leu

conostoc mesenteroides, and Propionibacterium

thoenii.

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BL Agar (Glucose Blood Liver Agar)