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Blood Agar Base No. 2 (BAM M22)

[所属分类:培养基配方] [发布时间:2021-11-5] [发布人:网站管理员2] [阅读次数:] [返回]

Blood Agar Base No. 2

(BAM M22)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1004mL:

Agar ..................................................................12.0g

Proteose peptone...............................................15.0g

NaCl....................................................................5.0g

Yeast extract........................................................5.0g

Liver digest .........................................................2.5g

Horse blood, defibrinated ............................. 50.0mL

FBP solution ................................................... 4.0mL

pH 7.4 ± 0.2 at 25°C

FBP Solution:

Composition per 30.0mL:

FeSO4................................................................0.25g

NaHSO3 ............................................................0.25g

Sodium pyruvate...............................................0.25g

Preparation of FBP Solution: Add components

to distilled/deionized water and bring volume to

30.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept horse blood and FBP solution, to distilled/deion

ized water and bring volume to 950.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

48°C. Aseptically add 50.0mL of sterile horse blood.

Mix thoroughly. Aseptically add 4.0mL sterile FBP

solution. Mix thoroughly. Pour into sterile Petri dish

es in 20.0mL volumes.

Use: For the cultivation of Brucella spp. and other

fastidious bacteria.