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Bolton Broth

[所属分类:培养基配方] [发布时间:2021-11-5] [发布人:网站管理员2] [阅读次数:] [返回]

Bolton Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 530mL:

Bolton selective enrichment broth base...... 500.0mL

Horse blood, lysed ........................................ 25.0mL

Bolton selective supplement solution ............. 5.0mL

pH 7.4 ± 0.2 at 25°C

Bolton Selective Enrichment Broth Base:

Composition per liter:

Peptone .............................................................10.0g

Lactalbumin hydrolysate ....................................5.0g

Yeast extract........................................................5.0g

NaCl....................................................................5.0g

a-ketoglutarate ....................................................1.0g

Na-pyruvate ........................................................0.5g

Na-metabisulfite .................................................0.5g

Na2CO3 ...............................................................0.6g

Hemin ...............................................................0.01g

Preparation of Bolton Selective Enrichment

Broth Base: Add components to distilled/deion

ized water and bring volume to 1.0L. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 50°C.

Bolton Broth Supplement Solution:

Composition per 5.0mL

Vancomycin .......................................... 10.0mg

Cefoperazone .................................................10.0mg

Trimethoprim.................................................10.0mg

Cycloheximide...............................................10.0mg

Ethanol ...................................................2.5mL:

Preparation of Bolton Supplement Solution:

Add antibiotics to 2.5mL ethanol. Mix thoroughly.

Bring volume to 5.0mL with distilled/deionized wa

ter. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine

500.0mL warm Bolton selective enrichment broth

base, 25.0mL lysed horse blood, and 5.0mL Bolton

selective supplement solution.

Use: For the enrichment of Campylobacter spp. from

foods.