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Brilliant Green Bile Agar

[所属分类:培养基配方] [发布时间:2021-11-5] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Noble agar.......................................................10.15g

Pancreatic digest of gelatin...............................8.25g

Lactose................................................................1.9g

Na2SO3............................................................0.205g

Basic Fuchsin..................................................0.078g

Erioglaucine....................................................0.065g

FeCl3 .............................................................0.0295g

KH2PO4...........................................................0.015g

Oxgall, dehydrated.........................................2.95mg

Brilliant Green ...............................................0.03mg

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Caution: Basic Fuchsin is a potential carcinogen

and care must be taken to avoid inhalation of the

powdered dye and contamination of the skin.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

For plating 10.0mL samples, prepare the medium

double strength. Mix thoroughly. Gently heat and

bring to boiling. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Pour into

sterile Petri dishes. Care should be taken to avoid ex

posure of the prepared medium to light.

Use: For the detection and enumeration of coliform

bacteria in materials of sanitary importance such as

water, sewage, and foods. Escherichia coli appears as

dark red colonies with a pink halo. Enterobacter spe

cies appear as pink colonies.