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Brilliant Green 2%-Bile Broth, Fluorocult® (Fluorocult Brilliant Green 2%-Bile Broth) (BRILA)

[所属分类:培养基配方] [发布时间:2021-11-5] [发布人:网站管理员2] [阅读次数:] [返回]

Brilliant Green 2%-Bile Broth,

Fluorocult®

(Fluorocult Brilliant Green

2%-Bile Broth)

(BRILA)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Ox bile, dried ....................................................20.0g

Peptone..............................................................10.0g

Lactose..............................................................10.0g

L-Tryptophan.......................................................1.0g

4-Methylumbelliferyl-β-D-glucuronide ..............0.1g

Brilliant Green ..............................................0.0133g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available from Merck.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Cool. Dis

tribute into into test tubes containing inverted Durham

tubes. Autoclave for 15 min at 15 psi pressure–121°C.

Do not autoclave longer. The prepared broth is clear

and green.

Use: For the cultivation of Escherichia coli. Bile and

brilliant green almost completely inhibit the growth

of undesired microbial flora, in particular Gram-pos

itive microorganisms. E. coli shows a positive fluo

rescence under UV light (366 nm). A positive indole

reaction and, if necessary gas formation due to fer

menting lactose, confirm the findings.