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Bromcresol Purple Broth with NaCl (BAM M26)

[所属分类:培养基配方] [发布时间:2021-11-9] [发布人:网站管理员2] [阅读次数:] [返回]

Bromcresol Purple Broth with NaCl

(BAM M26)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................25.0g

Peptone..............................................................10.0g

NaCl....................................................................5.0g

Beef extract.........................................................3.0g

Bromcresol Purple ............................................0.04g

Carbohydrate solution................................... 50.0mL

pH 7.0 ± 0.2 at 25°C

Carbohydrate Solution:

Composition per 50.0mL:

Carbohydrate.....................................................25.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 50.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 950.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 10

min at 15 psi pressure–121°C. Cool to 25°C. Asepti

cally add 50.0mL of carbohydrate solution. Asepti

cally distribute into tubes or flasks. Alternately

distribute the medium without the carbohydrate solu

tion into test tubes that contain an inverted Durham

tube prior to autoclaving. Then autoclave for 10 min

at 15 psi pressure–121°C, cool to 25°C, and asepti

cally add the carbohydrate solution to each tube to

yield a final carbohydrate concentration of 5%.

Use: For the differentiation of a halophilic Vibrio

spp. Bacteria that ferment the specific carbohydrate

turn the medium yellow. When bacteria produce gas,

the gas is trapped in the Durham tube.