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Bromcresol Purple Dextrose Broth (BCP Broth) (BAM M27)

[所属分类:培养基配方] [发布时间:2021-11-9] [发布人:网站管理员2] [阅读次数:] [返回]

Bromcresol Purple Dextrose Broth

(BCP Broth)

(BAM M27)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................10.0g

Peptone ...............................................................5.0g

Beef extract.........................................................3.0g

Bromcresol Purple solution ............................ 2.0mL

pH 7.0 ± 0.2 at 25°C

Bromcresol Purple Solution:

Composition per 10.0mL:

Bromcresol Purple ............................................0.16g

Ethanol (95% solution)................................. 10.0mL

Preparation of Bromcresol Purple Solution:

Add Bromcresol Purple to 10.0mL of ethanol. Mix

thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes in 12–15mL

volumes. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation and differentiation of bacte

ria based on their ability to ferment glucose. Bacteria

that ferment glucose turn the medium yellow.