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Bromthymol Blue Agar

[所属分类:培养基配方] [发布时间:2021-11-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................11.0g

Peptone .............................................................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................5.0g

Lactose (33% solution)................................. 27.0mL

Bromthymol Blue (1% solution) .................. 10.0mL

Sodium thiosulfate (50% solution) ................. 2.0mL

Glucose (33% solution) .................................. 1.2mL

Maranil solution (5% solution)....................... 1.0mL

pH 7.7–7.8 at 25°C

Preparation of Medium: Add agar, peptone,

NaCl, and yeast extract to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Adjust pH

to 8.0. Autoclave for 20 min at 15 psi pressure–121°C.

Cool to 45°–50°C. Filter sterilize the lactose solution,

Bromthymol Blue solution, sodium thiosulfate solu

tion, glucose solution, and maranil solution separately.

To the cooled, sterile agar solution, aseptically add

27.0mL of sterile lactose solution, 10.0mL of sterile

Bromthymol Blue solution, 2.0mL of sterile sodium

thiosulfate solution, 1.2mL of sterile glucose solution,

and 1.0mL of sterile maranil solution. Mix thoroughly.

Adjust pH to 7.7–7.8. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the selective isolation and cultivation of

members of the Enterobacteriaceae.