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Brucella Anaerobic Blood Agar

[所属分类:培养基配方] [发布时间:2021-11-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Vitamin K1 ........................................................0.01g

Anaerobic agar base.................................... 950.0mL

Sheep blood, sterile, defibrinated ................. 50.0mL

Anaerobic Agar Base

Composition per liter:

Pancreatic digest of casein................................17.5g

Agar ..................................................................15.0g

Glucose .............................................................10.0g

Papaic digest of soybean meal............................2.5g

NaCl....................................................................2.5g

Sodium thioglycolate ..........................................2.0g

Sodium formaldehyde sulfoxylate......................1.0g

L-Cystine·HCl·H2O .............................................0.4g

Methylene Blue...............................................0.002g

pH 7.0 ± 0.2 at 25°C

Preparation of Anaerobic Agar Base: Add

components to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Preparation of Medium: To 950.0mL of cooled,

sterile anaerobic agar base, aseptically add 10.0mg of

vitamin K1 and 50.0mL of sterile, defibrinated sheep

blood.

Use: For the isolation of anaerobes.