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Brucella Broth (Brucella Albimi Broth)

[所属分类:培养基配方] [发布时间:2021-11-9] [发布人:网站管理员2] [阅读次数:] [返回]

Brucella Broth

(Brucella Albimi Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................10.0g

Peptic digest of animal tissue............................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................2.0g

Glucose ...............................................................1.0g

NaHSO3 ..............................................................0.1g

Horse blood, defibrinated ............................. 50.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept horse blood, to distilled/deionized water and

bring volume to 950.0mL. Mix thoroughly. Heat gen

tly with frequent mixing. Boil for 1 min. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 45°–

50°C. Aseptically add 50.0mL of sterile horse blood.

Mix thoroughly. Aseptically distribute into sterile

tubes or flasks.

Use: For the cultivation and maintenance of Campy

lobacter coli, Campylobacter fecalis, and Brucella

species. Also used for the isolation and cultivation of

a wide variety of fastidious and nonfastidious micro

organisms.