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Brucella Medium, Selective

[所属分类:培养基配方] [发布时间:2021-11-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of casein................................10.0g

Peptic digest of animal tissue............................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................2.0g

Glucose ...............................................................1.0g

NaHSO3 ..............................................................0.1g

Horse serum ................................................ 100.0mL

VCNF antibiotic solution.............................. 10.0mL

pH 7.0 ± 0.2 at 25°C

Preparation of VCNF Antibiotic Solution:

Add components to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, ex

cept horse serum and VCNF antibiotic solution, to

distilled/deionized water and bring volume to

890.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add 100.0mL

of sterile horse serum and 10.0mL of VCNF antibiot

ic solution. Mix thoroughly. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the selective isolation, cultivation, and

maintenance of Brucella species.