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Brucella Selective Medium

[所属分类:培养基配方] [发布时间:2021-11-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, infusion from................................500.0g

Agar ..................................................................15.0g

Tryptose ............................................................10.0g

NaCl....................................................................5.0g

Glucose ...............................................................2.5g

Gelatin.................................................................1.0g

Sheep blood................................................. 100.0mL

Antibiotic solution ........................................ 10.0mL

pH 7.4 ± 0.2 at 25°C

Antibiotic Solution:

Composition per 10.0mL:

Cycloheximide....................................................1.0g

Bacitracin...................................................250,000U

Circulin ......................................................250,000U

Polymyxin B ..............................................100,000U

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept sheep blood and antibiotic solution, to distilled/

deionized water and bring volume to 890.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add 100.0mL of sterile sheep

blood and 10.0mL of sterile antibiotic solution. Mix

thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the selective isolation and cultivation of

Brucella species.