培养基配方

您现在的位置：网站首页 >> 培养基配方

Campylobacter Agar, Skirrow’s (Skirrow’s Campylobacter Agar)

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

Campylobacter Agar, Skirrow’s

(Skirrow’s Campylobacter Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Campylobacter agar base............................ 990.0mL

Supplement S................................................ 10.0mL

pH 7.4 ± 0.2 at 25°C

Campylobacter Agar Base:

Composition per liter:

Proteose peptone...............................................15.0g

Agar ..................................................................12.0g

NaCl....................................................................5.0g

Yeast extract........................................................5.0g

Liver digest .........................................................2.5g

Source: Campylobacter agar base and Campylo

bacter antimicrobic supplement S are available as a

premixed powder from BD Diagnostic Systems.

Preparation of Campylobacter Agar Base:

Add components to distilled/deionized water and

bring volume to 990.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C.

Supplement S:

Composition per 10.0mL:

Vancomycin ...................................................10.0mg

Trimethoprim ...................................................5.0mg

Polymyxin B ...................................................2500U

Preparation of Supplement S: Add components

to 10.0mL of distilled/deionized water. Filter sterilize.

Preparation of Medium: Prepare 990.0mL of

Campylobacter agar base. Autoclave and cool to 45°–

50°C. Aseptically add 10.0mL of sterile supplement S.

Mix thoroughly. Pour into sterile Petri dishes.

Use: For the selective isolation of Campylobacterje

juni from foods.