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Campylobacter Charcoal Differential Agar (CCDA) (Preston Blood-Free Medium)

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

Campylobacter Charcoal

Differential Agar

(CCDA)

(Preston Blood-Free Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................12.0g

Beef extract.......................................................10.0g

Peptone .............................................................10.0g

NaCl....................................................................5.0g

Charcoal..............................................................4.0g

Casein hydrolysate..............................................3.0g

Sodium deoxycholate..........................................1.0g

FeSO4................................................................0.25g

Sodium pyruvate...............................................0.25g

Cefoperazone solution .................................. 10.0mL

pH 7.5 ± 0.2 at 25°C

Cefoperazone Solution:

Composition per 10.0mL:

Sodium cefoperazone......................................0.032g

Preparation of Cefoperazone Solution: Add

sodium cefoperazone to distilled/deionized water

and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, ex

cept cefoperazone solution, to distilled/deionized

water and bring volume to 990.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 10.0mL of sterile cefoperazone solu

tion. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the cultivation of Campylobacter species.