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Campylobacter Blood-Free Selective Agar

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

Campylobacter Blood-Free

Selective Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................12.0g

Beef extract.......................................................10.0g

Peptone .............................................................10.0g

Charcoal..............................................................4.0g

Casein hydrolysate..............................................3.0g

Sodium deoxycholate..........................................1.0g

Fe2SO4·H2O ......................................................0.25g

Sodium pyruvate...............................................0.25g

Cefoperazone solution .................................. 10.0mL

pH 7.4 ± 0.2 at 25°C

Cefoperazone Solution:

Composition per 10.0mL:

Sodium cefoperazone......................................0.032g

Preparation of Cefoperazone Solution: Add

sodium cefoperazone to distilled/deionized water

and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, ex

cept cefoperazone solution, to distilled/deionized

water and bring volume to 990.0mL. Mix thoroughly.

Heat with frequent agitation and boil for 1 min to

completely dissolve. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°–55°C. Add 10.0mL of

sterile cefoperazone solution. Addition of 10.0mg/L

of amphotericin B improves the selectivity of the me

dium. Mix thoroughly. Pour into sterile Petri dishes.

Use: For the selective isolation of Campylobacter

species, especially Campylobacter jejuni, Campylo

bacter coli, and Campylobacter laridis.