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Campylobacter Isolation Agar A

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................12.0g

Beef extract.......................................................10.0g

Peptone..............................................................10.0g

NaCl....................................................................5.0g

Charcoal..............................................................4.0g

Casein hydrolysate..............................................3.0g

Yeast extract........................................................2.0g

Sodium deoxycholate..........................................1.0g

FeSO4 ................................................................0.25g

Sodium pyruvate...............................................0.25g

Antibiotic solution ........................................ 10.0mL

pH 7.4 ± 0.2 at 25°C

Antibiotic Solution:

Composition per 10.0mL:

Cycloheximide....................................................0.1g

Sodium cefoperazone........................................0.03g

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept antibiotic solution, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptical

ly add sterile antibiotic solution. Mix thoroughly.

Pour into sterile Petri dishes. Swirl flask while pour

ing to distribute charcoal.

Use: For the isolation and cultivation of Campylo

bacter species.