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Campylobacter Isolation Agar B (Campy Cefex Agar)

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

Campylobacter Isolation Agar B

(Campy Cefex Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of casein................................10.0g

Peptic digest of animal tissue ...........................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................2.0g

Glucose ...............................................................1.0g

FeSO4..................................................................0.5g

Sodium pyruvate.................................................0.5g

NaHSO3 ............................................................0.35g

Horse blood, laked........................................ 50.0mL

Antibiotic solution ........................................ 10.0mL

pH 7.0 ± 0.2 at 25°C

Horse Blood, Laked:

Composition per 50.0mL:

Horse blood, fresh......................................... 50.0mL

Preparation of Horse Blood, Laked: Add

blood to a sterile polypropylene bottle. Freeze over

night at −20°C. Thaw at 8°C. Refreeze at −20°C.

Thaw again at 8°C.

Antibiotic Solution:

Composition per 10.0mL:

Cycloheximide....................................................0.1g

Sodium cefoperazone......................................0.033g

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept horse blood and antibiotic solution, to distilled/

deionized water and bring volume to 940.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile horse blood and

antibiotic solution. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Campylo

bacter species.