编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

Campylobacter Medium

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium aspartate...............................................10.0g

MgSO4·7H2O..................................................... 1.0g

K2HPO4.............................................................0.75g

Yeast extract........................................................0.2g

CaCl2·2H2O ...................................................28.0mg

Resazurin .........................................................1.0mg

Phosphate-cysteine solution........................ 100.0mL

Trace elements solution SL-10 ....................... 1.0mL

pH 7.0 ± 0.2 at 25°C

Phosphate-Cysteine Solution:

Composition per 100.0mL:

NaH2PO4 ...........................................................0.25g

L-Cysteine·HCl .................................................0.25g

Preparation of Phosphate-Cysteine Solution:

Add components to distilled/deionized water and

bring volume to 100.0mL. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C.

Trace Elements Solution SL-10:

Composition per liter:

FeCl2·4H2O .........................................................1.5g

CoCl2·6H2O .................................................190.0mg

MnCl2·4H2O ................................................100.0mg

ZnCl2..............................................................70.0mg

Na2MoO4·2H2O .............................................36.0mg

NiCl2·6H2O ....................................................24.0mg

H3BO3 ..............................................................6.0mg

CuCl2·2H2O .....................................................2.0mg

HCl (25% solution)....................................... 10.0mL

Preparation of Trace Elements Solution SL-10:

Add FeCl2·4H2O to 10.0mL of HCl solution. Mix

thoroughly. Add distilled/deionized water and bring

volume to 1.0L. Add remaining components. Mix

thoroughly.

Preparation of Medium: Add components, ex

cept phosphate-cysteine solution, to distilled/deion

ized water and bring volume to 900.0mL. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C. Aseptically add 100.0mL of sterile phos

phate-cysteine solution. Mix thoroughly. Aseptically

distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Campy

lobacter species.

在线客服

Campylobacter Medium