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Campylobacter Selective Medium, Blaser-Wang (Blaser-Wang Campylobacter Medium) (Blaser’s Agar) (Campy BAP Medium)

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

Campylobacter Selective

Medium, Blaser-Wang

(Blaser-Wang Campylobacter Medium)

(Blaser’s Agar)

(Campy BAP Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Brucella agar base....................................... 890.0mL

Sheep blood................................................. 100.0mL

Antibiotic supplement................................... 10.0mL

Brucella Agar Base:

Composition per 890.0mL:

Agar ..................................................................15.0g

Glucose .............................................................10.0g

Pancreatic digest of casein................................10.0g

NaCl....................................................................5.0g

Peptic digest of animal tissue .............................5.0g

pH 7.5 ± 0.2 at 25°C

Preparation of Brucella Agar Base: Add com

ponents to distilled/deionized water and bring vol

ume to 890.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C.

Antibiotic Supplement:

Composition per 10.0mL:

Cephalothin....................................................15.0mg

Vancomycin ...................................................10.0mg

Trimethoprim...................................................5.0mg

Amphotericin B ...............................................2.0mg

Polymyxin B ...................................................2500U

Preparation of Antibiotic Supplement: Add

components to 10.0mL of distilled/deionized water.

Filter sterilize.

Preparation of Medium: Prepare 890.0mL of

Brucella agar base. Sterilize as directed. Cool to 50°–

55°C and add 100.0mL of sheep blood or 50.0–

70.0mL of laked horse blood. Laked blood is pre

pared by freezing whole blood overnight and thaw

ing to room temperature. Aseptically add 10.0mL of

sterile antibiotic supplement. Mix thoroughly. Pour

into sterile Petri dishes.

Use: For the selective isolation of Campylobacter

species.

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Campylobacter Selective Medium, Blaser-Wang (Blaser-Wang Campylobacter Medium) (Blaser’s Agar) (Campy BAP Medium)