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Campylobacter Selective Medium, Blaser-Wang (Blaser-Wang Campylobacter Medium)

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

Campylobacter Selective

Medium, Blaser-Wang

(Blaser-Wang Campylobacter Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Columbia agar base..................................... 890.0mL

Sheep blood ................................................ 100.0mL

Antibiotic supplement................................... 10.0mL

pH 7.3 ± 0.2 at 25°C

Columbia Agar Base:

Composition per liter:

Special peptone.................................................25.0g

Agar ..................................................................10.0g

NaCl....................................................................5.0g

Starch ..................................................................1.0g

Preparation of Columbia Agar Base: Add

components to distilled/deionized water and bring

volume to 890.0mL. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C.

Antibiotic Supplement:

Composition per 10.0mL:

Cephalothin....................................................15.0mg

Vancomycin ...................................................10.0mg

Trimethoprim ...................................................5.0mg

Amphotericin B................................................2.0mg

Polymyxin B ..................................................2,500U

Preparation of Antibiotic Supplement: Add

components to 10.0mL of distilled/deionized water.

Filter sterilize.

Preparation of Medium: To 890.0mL of cooled,

sterile Columbia agar base, aseptically add 100.0mL

of sheep blood or 50.0–70.0mL of laked horse blood.

Laked blood is prepared by freezing whole blood

overnight and thawing to room temperature. Asepti

cally add 10.0mL of sterile antibiotic supplement.

Mix thoroughly. Pour into sterile Petri dishes.

Use: For the selective isolation of Campylobacter

species.