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Campylobacter Selective Medium, Butzler’s (Butzler’s Campylobacter Medium)

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

Campylobacter Selective

Medium, Butzler’s

(Butzler’s Campylobacter Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Brucella agar base....................................... 940.0mL

Sheep or horse blood, defibrinated ............... 50.0mL

Antibiotic supplement................................... 10.0mL

pH 7.5 ± 0.2 at 25°C

Brucella Agar Base:

Composition per liter:

Agar ..................................................................15.0g

Glucose .............................................................10.0g

Pancreatic digest of casein................................10.0g

NaCl....................................................................5.0g

Peptic digest of animal tissue..............................5.0g

Preparation of Brucella Agar Base: Add com

ponents to distilled/deionized water and bring vol

ume to 940.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C.

Antibiotic Supplement:

Composition per 10.0mL:

Cycloheximide...............................................50.0mg

Cephazolin .....................................................15.0mg

Novobiocin.......................................................5.0mg

Bacitracin.....................................................25,000U

Colistin sulfate .............................................10,000U

Preparation of Antibiotic Supplement: Add

components to 10.0mL of distilled/deionized water.

Filter sterilize.

Preparation of Medium: To 940.0mL of cooled,

sterile Brucella agar base, aseptically add 50.0mL of

defibrinated sheep or horse blood and 10.0mL of

sterile antibiotic supplement. Mix thoroughly. For

enhanced growth, medium may also be supplement

ed with 0.25g of Fe2SO4·H2O, 0.25g of sodium met

abisulfite, and 0.25g of sodium pyruvate. Pour into

sterile Petri dishes.

Use: For the selective isolation of Campylobacter

species.