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Campylobacter Selective Medium, Preston’s (Preston’s Campylobacter Medium)

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

Campylobacter Selective Medium,

Preston’s

(Preston’s Campylobacter Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Campylobacter agar base............................ 940.0mL

Horse blood, lysed ........................................ 50.0mL

Antibiotic supplement................................... 10.0mL

pH 7.5 ± 0.2 at 25°C

Campylobacter Agar Base:

Composition per liter:

Agar ..................................................................12.0g

Beef extract.......................................................10.0g

Peptone .............................................................10.0g

NaCl....................................................................5.0g

Preparation of Campylobacter Agar Base:

Add components to distilled/deionized water and

bring volume to 940.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C.

Antibiotic Supplement:

Composition per 10.0mL:

Cycloheximide....................................................0.1g

Rifampicin ........................................................0.01g

Trimethoprim lactate.........................................0.01g

Polymyxin B ...................................................5000U

Preparation of Antibiotic Supplement: Add

components to 10.0mL of 50:50 acetone:distilled/

deionized water. Filter sterilize.

Preparation of Medium: To 940.0mL of cooled,

sterile Campylobacter agar base, aseptically add

50.0mL of lysed horse blood and 10.0mL of sterile

antibiotic supplement. Mix thoroughly. Pour into

sterile Petri dishes.

Use: For the selective isolation of Campylobacter

species.