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Carbohydrate Fermentation Broth

[所属分类:培养基配方] [发布时间:2021-11-10] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .............................................................10.0g

NaCl....................................................................5.0g

Meat extract ........................................................3.0g

Carbohydrate solution................................... 50.0mL

Andrade’s indicator....................................... 10.0mL

pH 7.1 ± 0.2 at 25°C

Andrade’s Indicator:

Composition per 100.0mL:

Acid Fuchsin.......................................................0.1g

NaOH (1N solution)...................................... 16.0mL

Preparation of Andrade’s Indicator: Add

components to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly.

Carbohydrate Solution:

Composition per 100.0mL:

Carbohydrate.....................................................10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 100.0mL. Adonitol, arabinose, cellobiose,

glucose, dulcitol, fructose, galactose, inositol, lac

tose, maltose, mannitol, raffinose, rhamnose, salicin,

sorbitol, sucrose, trehalose, xylose, or other carbohy

drates may be used. Mix thoroughly. Filter sterilize.

Caution: Acid Fuchsin is a potential carcinogen

and care must be taken to avoid inhalation of the

powdered dye and contact with the skin.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly. Gently

heat and bring to boiling. Distribute in 10.0mL vol

umes into test tubes containing inverted Durham

tubes. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 25°C. Add 0.5mL of sterile carbohy

drate solution to each tube.

Use: For the determination of carbohydrate fermen

tation reactions of microorganisms, particularly

members of the Enterobacteriaceae. A Durham tube

is used to collect gas produced during the fermenta

tion reaction. Acid production is indicated by a pink

reaction.

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Carbohydrate Fermentation Broth