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Casamino Acids Yeast Extract Lincomycin Medium

[所属分类:培养基配方] [发布时间:2021-11-11] [发布人:网站管理员2] [阅读次数:] [返回]

Casamino Acids Yeast

Extract Lincomycin Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Casamino acids .................................................20.0g

K2HPO4 ............................................................8.71g

Yeast extract........................................................6.0g

NaCl....................................................................2.5g

Lincomycin solution ....................................... 5.0mL

Trace salts solution ......................................... 1.0mL

pH 8.5 ± 0.2 at 25°C

Lincomycin Solution:

Composition per 5.0mL:

Lincomycin....................................................45.0mg

Preparation of Lincomycin Solution: Add lin

comycin to distilled/deionized water and bring vol

ume to 5.0mL. Mix thoroughly. Filter sterilize.

Trace Salts Solution:

Composition per liter:

MgSO4·7H2O....................................................50.0g

MnCl2· 4H2O ......................................................5.0g

FeCl2 ...................................................................5.0g

Preparation of Trace Salts Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 1.0L. Add sufficient 0.1N H2SO4 to dissolve

components. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept trace salts solution and lincomycin solution, to

distilled/deionized water and bring volume to

994.0mL. Mix thoroughly. Adjust pH to 8.5. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

25°C. Aseptically add 1.0mL of sterile trace salts so

lution and 5.0mL of sterile lincomycin solution. Mix

thoroughly. Aseptically distribute into sterile tubes or

flasks.

Use: For the cultivation of heat-labile, toxin-produc

ing enterotoxigenic Escherichia coli.