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Casamino Acids Yeast Extract Salts Broth, Gorbach (CA YE Broth) (BAM M34)

[所属分类:培养基配方] [发布时间:2021-11-11] [发布人:网站管理员2] [阅读次数:] [返回]

Casamino Acids Yeast

Extract Salts Broth, Gorbach

(CA YE Broth)

(BAM M34)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Casamino acids .................................................20.0g

K2HPO4 ............................................................8.71g

Yeast extract........................................................6.0g

NaCl....................................................................2.5g

Trace salts solution ......................................... 1.0mL

pH 8.5 ± 0.2 at 25°C

Trace Salts Solution:

Composition per liter:

MgSO4·7H2O ....................................................50.0g

MnCl2· 4H2O ......................................................5.0g

FeCl2 ...................................................................5.0g

Preparation of Trace Salts Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 1.0L. Add sufficient 0.1N H2SO4 to dissolve

components. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept trace salts solution, to distilled/deionized water

and bring volume to 999.0mL. Mix thoroughly. Ad

just pH to 8.5. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 25°C. Aseptically add 1.0mL of

sterile trace salts solution. Mix thoroughly. Asepti

cally distribute into sterile tubes or flasks.

Use: For the cultivation of enterotoxigenic Escheri

chia coli.