培养基配方

您现在的位置：网站首页 >> 培养基配方

CCY Modified Medium

[所属分类:培养基配方] [发布时间:2021-11-11] [发布人:网站管理员2] [阅读次数:] [返回]

CCY Modified Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract..................................................... 30.0g

Casamino acids .................................................20.0g

Na2HPO4 ..........................................................2.48g

KH2PO4.............................................................0.41g

MgSO4·7H2O .................................................20.0mg

MnSO4·H2O .....................................................7.5mg

Citric acid.........................................................6.4mg

FeSO4·7H2O.....................................................6.4mg

Sodium pyruvate solution ......................... 100.00mL

pH 7.3 ± 0.2 at 25°C

Sodium Pyruvate Solution:

Composition per 100.0mL:

Sodium pyruvate...............................................23.2g

Preparation of Sodium Pyruvate Solution:

Add sodium pyruvate to distilled/deionized water

and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, ex

cept sodium pyruvate solution, to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly.

Adjust pH to 7.3. Autoclave for 15 min at 15 psi pres

sure–121°C. Aseptically add 100.0mL of sterile sodi

um pyruvate solution. Mix thoroughly. Aseptically

distribute into sterile tubes or flasks.

Use: For the cultivation of Staphylococcus aureus.