编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

Cell Growth Medium (BAM M36)

[所属分类:培养基配方] [发布时间:2021-11-11] [发布人:网站管理员2] [阅读次数:] [返回]

Cell Growth Medium

(BAM M36)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 2250.0mL:

Eagle’s minimal essential medium

with Hanks’ salts (MEMH) ......................... 1.0L

L 15 medium, modified Leibovitz..................... 1.0L

Fetal calf serum........................................... 200.0mL

NaHCO3 solution.......................................... 50.0mL

pH 7.5 at 25°C

MEMH:

Composition per liter:

NaCl....................................................................8.0g

Glucose ...............................................................1.0g

KCl......................................................................0.4g

CaCl2·2H2O ......................................................0.14g

MgSO4·7H2O......................................................0.1g

KH2PO4.............................................................0.06g

Na2HPO4...........................................................0.05g

L-Isoleucine.....................................................0.026g

L-Leucine ........................................................0.026g

L-Lysine...........................................................0.026g

L-Threonine.....................................................0.024g

L-Valine .........................................................0.0235g

L-Tyrosine .......................................................0.018g

L-Arginine .....................................................0.0174g

L-Phenylalanine.............................................0.0165g

L-Cystine .........................................................0.012g

L-Histidine........................................................8.0mg

L-Methionine....................................................7.5mg

Phenol Red.......................................................5.0mg

L-Tryptophan....................................................4.0mg

Inositol .............................................................1.8mg

Biotin ...............................................................1.0mg

Folic acid .........................................................1.0mg

Calcium pantothenate ......................................1.0mg

Choline chloride...............................................1.0mg

Nicotinamide....................................................1.0mg

Pyridoxal·HCl ..................................................1.0mg

Thiamine·HCl ..................................................1.0mg

Riboflavin ........................................................0.1mg

Preparation of MEMH: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Filter sterilize.

L 15 Medium, Modified Leibovitz

Composition per liter:

NaCl....................................................................8.0g

DL-Threonine.......................................................0.6g

Sodium pyruvate.................................................0.6g

DL-Alanine...........................................................0.5g

L-Arginine, free base ...........................................0.5g

KCl......................................................................0.4g

L-Asparagine·H2O ...............................................0.3g

L-Histidine, free base...........................................0.3g

L-Glutamine.........................................................0.3g

L-Isoleucine .........................................................0.3g

L-Phenylalanine...................................................0.3g

L-Tyrosine............................................................0.3g

DL-Methionine.....................................................0.2g

DL-Valine .............................................................0.2g

Glycine................................................................0.2g

L-Serine ...............................................................0.2g

Na2HPO4, anhydrous ..........................................0.2g

CaCl2, anhydrous ................................................0.1g

L-Cysteine, free base ...........................................0.1g

L-Leucine·HCl.....................................................0.1g

Streptomycin.......................................................0.1g

MgCl2, anhydrous ...........................................0.094g

D-Galactose .......................................................0.09g

KH2PO4.............................................................0.06g

Gentamicin........................................................0.05g

L-Tryptophan.....................................................0.02g

Phenol Red........................................................0.01g

i-Inositol...........................................................2.0mg

Choline chloride...............................................1.0mg

D-Calcium pantothenate ...................................1.0mg

Folic acid..........................................................1.0mg

Nicotinamide....................................................1.0mg

Pyridoxine·HCl ................................................1.0mg

Thiamine monophosphate·2H2O......................1.0mg

Riboflavin-5-phosphate ...................................0.1mg

Penicillin G ................................................100,000U

pH 7.5 ± 0.2 at 25°C

Preparation of L 15 Medium, Modified Lei

bovitz: Add components to distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly. Filter

sterilize. Store at 5°C.

NaHCO3 Solution:

Composition per 100.0mL:

NaHCO3 ..............................................................7.5g

Preparation of NaHCO3 Solution: Add

NaHCO3 to distilled/deionized water and bring vol

ume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine

1.0L of sterile Eagle’s minimal essential medium

with Hanks’ salts (MEMH) and 1.0L of sterile L 15

medium, modified Leibovitz. Mix thoroughly. Im

mediately prior to use, aseptically add 200.0mL of

fetal calf serum and 50.0mL of NaHCO3 solution.

Mix thoroughly. Aseptically distribute into sterile

containers.

Use: For the cultivation of mammalian HeLa or Vero

tissue culture cells to test the cytopathic effects of Es

cherichia coli.

在线客服

Cell Growth Medium (BAM M36)