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Cellobiose Polymyxin Colistin Agar (CPC Agar)

[所属分类:培养基配方] [发布时间:2021-11-11] [发布人:网站管理员2] [阅读次数:] [返回]

Cellobiose Polymyxin Colistin Agar

(CPC Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A................................................... 900.0mL

Solution B ................................................... 100.0mL

pH 7.6 ± 0.2 at 25°C

Solution A:

Composition per 900.0mL:

NaCl..................................................................20.0g

Agar ..................................................................15.0g

Peptone .............................................................10.0g

Beef extract.........................................................5.0g

Bromthymol Blue .............................................0.04g

Cresol Red ........................................................0.04g

Preparation of Solution A: Add components to

distilled/deionized water and bring volume to

900.0mL. Mix thoroughly. Adjust pH to 7.6. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 50°–55°C.

Solution B:

Composition per 100.0mL:

Cellobiose .........................................................15.0g

Colistin....................................................1,360,000U

Polymyxin B ..............................................100,000U

Preparation of Solution B: Add components to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine

900.0mL of cooled, sterile solution A and 100.0mL

of sterile solution B. Mix thoroughly. Pour into ster

ile Petri dishes. Use within 7 days.

Use: For the cultivation and identification of Vibrio

species from foods.