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Cellobiose Polymyxin B Colistin Agar, Modified (mCPC Agar) (BAM M98)

[所属分类:培养基配方] [发布时间:2021-11-11] [发布人:网站管理员2] [阅读次数:] [返回]

Cellobiose Polymyxin B

Colistin Agar, Modified

(mCPC Agar)

(BAM M98)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution 1.................................................... 900.0mL

Solution 2.................................................... 100.0mL

pH 7.6 ± 0.2 at 25°C

Solution 1:

Composition per 900.0mL:

NaCl..................................................................20.0g

Agar ..................................................................15.0g

Peptone..............................................................10.0g

Beef extract.........................................................5.0g

1000× dye stock solution ................................ 1.0mL

Preparation of Solution 1: Add components to

distilled/deionized water and bring volume to

900.0mL. Mix thoroughly. Adjust pH to 7.6. Gently

heat and bring to boiling. Do not autoclave. Cool to

48°–55°C.

1000X Dye Stock Solution:

Composition per 100.0mL:

Bromthymol Blue ...............................................4.0g

Cresol Red...........................................................4.0g

Ethanol (95% solution) ............................... 100.0mL

Preparation of 1000X Dye Stock Solution:

Add Bromthymol Blue and Cresol Red to 100.0mL

of ethanol. Mix thoroughly.

Solution 2:

Composition per 100.0mL:

Cellobiose .........................................................10.0g

Colistin.......................................................400,000U

Polymyxin B ..............................................100,000U

Preparation of Solution 2: Add cellobiose to dis

tilled/deionized water and bring volume to 100.0mL.

Mix thoroughly. Gently heat until dissolved. Cool to

25°C. Add colistin and polymyxin B. Mix thoroughly.

Preparation of Medium: Combine cooled solu

tion 1 and solution 2. Mix thoroughly. Do not auto

clave. Pour into sterile Petri dishes.

Use: For the cultivation of Vibrio species from

foods.

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Cellobiose Polymyxin B Colistin Agar, Modified (mCPC Agar) (BAM M98)