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Cholera Medium TCBS

[所属分类:培养基配方] [发布时间:2021-11-11] [发布人:网站管理员2] [阅读次数:] [返回]

Cholera Medium TCBS

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose..............................................................20.0g

Agar ..................................................................14.0g

Peptone..............................................................10.0g

NaCl..................................................................10.0g

Sodium citrate...................................................10.0g

Na2S2O3·5H2O ..................................................10.0g

Ox bile.................................................................8.0g

Yeast extract........................................................5.0g

Ferric citrate........................................................1.0g

Bromthymol Blue .............................................0.04g

Thymol Blue .....................................................0.04g

pH 8.6 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling. Do

not autoclave. Pour into sterile Petri dishes. Dry agar

plates before using.

Use: For the growth of Vibrio cholerae, Vibrio para

haemolyticus, and other Vibrio species.