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Chopped Liver Broth (BAM M38)

[所属分类:培养基配方] [发布时间:2021-11-11] [发布人:网站管理员2] [阅读次数:] [返回]

Chopped Liver Broth

(BAM M38)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Fresh beef liver ...............................................500.0g

Peptone..............................................................10.0g

K2HPO4 ..............................................................1.0g

Soluble starch......................................................1.0g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Grind fresh beef liver.

Add to 1.0L of distilled/deionized water. Gently heat

and bring to boiling. Continue boiling for 60 min.

Cool to 25°C. Adjust pH to 7.0. Gently heat and bring

to boiling. Continue boiling for 10 min. Filter

through cheesecloth. Press out excess liquid. Save

chopped liver particles. To filtrate, add remaining

components. Bring volume to 1.0L with distilled/

deionized water. Adjust pH to 7.0. Filter through

Whatman #1 filter paper. Add chopped liver particles

to test tubes to a depth of 1.2–2.5 cm. Add 10.0mL of

broth to each tube. Autoclave for 15 min at 15 psi

pressure–121°C.

Use: For the isolation and cultivation of Clostridium

botulinum, Clostridium perfringens, and other anaer

obic bacteria from foods.