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Chopped Meat Agar

[所属分类:培养基配方] [发布时间:2021-11-11] [发布人:网站管理员2] [阅读次数:] [返回]

Chopped Meat Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Ground meat, fat free......................................500.0g

Pancreatic digest of casein................................30.0g

Agar ..................................................................15.0g

K2HPO4...............................................................5.0g

Yeast extract........................................................5.0g

L-Cysteine·HCl ...................................................0.5g

Resazurin .........................................................1.0mg

NaOH (1N solution)...................................... 25.0mL

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Use lean beef or horse

meat. Remove fat and connective tissue. Grind fine

ly. Add ground meat and 25.0mL of NaOH solution

to distilled/deionized water and bring volume to

1025mL. Gently heat and bring to boiling. Continue

boiling for 15 min without stirring. Cool to room

temperature. Remove fat from surface. Filter and re

tain both meat particles and filtrate. Adjust volume of

filtrate to 1.0L with distilled/deionized water. Add

pancreatic digest of casein, agar, K2HPO4, yeast ex

tract, and resazurin. Gently heat and bring to boiling.

Boil for 1–2 min. Add L-cysteine·HCl. Mix thor

oughly. Distribute 7.0mL into tubes that contain meat

particles (1 part meat particles to 5 parts fluid). Auto

clave for 30 min at 15 psi pressure–121°C.

Use: For the cultivation of various anaerobes.