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Chromogenic Substrate Broth

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................10.0g

HEPES (N-[2-Hydroxyethyl]

piperazine-N′-[2-ethane

sulfonic acid]) buffer....................................6.9g

(NH4)2SO4 ..........................................................5.0g

o-Nitrophenyl-β-D-galactopyranoside.................0.5g

Solanium .............................................................0.5g

MgSO4 ................................................................0.1g

4-Methylumbelliferyl-β-D-glucuronide...........0.075g

CaCl2.................................................................0.05g

Na2SO3..............................................................0.04g

Amphotericin B................................................1.0mg

MnSO4 .............................................................0.5mg

ZnSO4 ..............................................................0.5mg

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the detection of coliform bacteria based on

their hydrolysis of chromogenic substrates by produc

tion of β-D-galactopyranosidase. Bacteria that produce

β-D-galactopyranosidase turn the medium yellow.