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CIN Agar (Yersinia Selective Agar) (Cefsulodin Irgasan Novobiocin Agar)

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

CIN Agar

(Yersinia Selective Agar)

(Cefsulodin Irgasan Novobiocin Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Mannitol............................................................20.0g

Agar ..................................................................12.0g

Pancreatic digest of gelatin...............................10.0g

Beef extract.........................................................5.0g

Peptic digest of animal tissue..............................5.0g

Sodium pyruvate.................................................2.0g

Yeast extract........................................................2.0g

NaCl....................................................................1.0g

Sodium deoxycholate..........................................0.5g

Neutral Red.......................................................0.03g

Cefsulodin.......................................................0.015g

Irgasan (triclosan) ............................................4.0mg

Novobiocin.......................................................2.5mg

Crystal Violet ...................................................1.0mg

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept cefsulodin and novobiocin, to distilled/deion

ized water and bring volume to 1.0L. Heat, mixing

continuously, until boiling. Do not autoclave. Cool to

45°–50°C. Aseptically add cefsulodin and novobio

cin. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the selective isolation and differentiation of

Yersinia enterocolitica based on mannitol fermenta

tion. Yersinia enterocolitica appears as “bull’s eye”

colonies with deep red centers surrounded by a trans

parent periphery.