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Clostridium CK Medium (DSMZ Medium 869)

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

Clostridium CK Medium

(DSMZ Medium 869)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..................................3.2g

NaCl....................................................................0.9g

Papaic digest of soybean meal............................0.6g

K2HPO4...............................................................0.5g

Glucose ...............................................................0.5g

Resazurin .........................................................0.5mg

Glucose solution ........................................... 10.0mL

pH 5.5 ± 0.2 at 25°C

Glucose Solution:

Composition per 10.0mL:

Glucose..............................................................2.0g

Preparation of Glucose Solution: Add glucose

to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Sparge with 100% N2. Fil

ter sterilize.

Preparation of Medium: Add components, ex

cept glucose solution, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Ad

just pH to 5.5. Sparge with 100% N2. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 25°C.

Aseptically and anaerobically add 10.0mL sterile

glucose solution. Aseptically and anaerobically dis

tribute into tubes or flasks.

Use: For the cultivation of Clostridium akagii,

Clostridium acidisoli, and Clostridium uliginosum.