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Clostridium difficile Agar

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Clostridum difficile agar base ..................... 920.0mL

Clostridium difficile

selective supplement............................... 10.0mL

Horse blood, defibrinated ............................. 70.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Clostridium difficile Agar Base:

Composition per 920.0mL:

Proteose peptone...............................................40.0g

Agar ..................................................................15.0g

Fructose...............................................................6.0g

Na2HPO4.............................................................5.0g

NaCl....................................................................2.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O......................................................0.1g

Preparation of Clostridium difficile Agar

Base: Add components to distilled/deionized water

and bring volume to 920.0mL. Mix thoroughly. Gen

tly heat to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C.

Clostridium difficile Selective Supplement:

Composition per 10.0mL:

D-Cycloserine...............................................500.0mg

Cefoxitin ........................................................16.0mg

Preparation of Clostridium difficile Selective

Supplement: Add components to distilled/deion

ized water and bring volume to 10.0mL. Mix thor

oughly. Filter sterilize.

Preparation of Medium: Add 10.0mL of sterile

Clostridium difficile selective supplement and

70.0mL of sterile, defibrinated horse blood to

920.0mL of cooled, sterile Clostridium difficile agar

base. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the selective isolation and cultivation of

Clostridium difficile.