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Clostridium difficile Agar (Cycloserine Cefoxitin Fructose Agar) (CCFA)

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

Clostridium difficile Agar

(Cycloserine Cefoxitin Fructose Agar)

(CCFA)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptic digest of animal tissue ...........................32.0g

Agar ..................................................................20.0g

Fructose...............................................................6.0g

Na2HPO4.............................................................5.0g

NaCl....................................................................2.0g

KH2PO4...............................................................1.0g

Cycloserine .......................................................0.25g

MgSO4 ................................................................0.1g

Neutral Red.......................................................0.03g

Cefoxitin solution ......................................... 10.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Cefoxitin Solution:

Composition per 10.0mL:

Cefoxitin ........................................................16.0mg

Preparation of Cefoxitin Solution: Add cefox

itin to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to

990.0mL. Mix thoroughly. Gently heat to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C. Aseptically add 10.0mL of sterile ce

foxitin solution. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the selective isolation and cultivation of

Clostridium difficile.