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Clostridium perfringens Agar, OPSP (Perfringens Agar, OPSP)

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

Clostridium perfringens Agar, OPSP

(Perfringens Agar, OPSP)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................15.0g

Agar ..................................................................10.0g

Liver extract........................................................7.0g

Papaic digest of soybean meal............................5.0g

Yeast extract........................................................5.0g

Tris(hydroxymethyl)aminomethane buffer.........1.5g

Ferric ammonium citrate.....................................1.0g

Na2S2O5 ..............................................................1.0g

Antibiotic inhibitor ....................................... 10.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Antibiotic Inhibitor:

Composition per 10.0mL:

Sodium sulfadiazine............................................0.1g

Oleandomycin phosphate.................................0.5mg

Polymyxin B ................................................10,000U

Preparation of Antibiotic Inhibitor: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except

antibiotic inhibitor, to distilled/deionized water and

bring volume to 990.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add sterile antibiotic inhibitor. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the presumptive identification and enumer

ation of Clostridium perfringens in foods.