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Clostridium Selective Agar (Clostrisel Agar)

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

Clostridium Selective Agar

(Clostrisel Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................17.0g

Agar ..................................................................14.0g

Glucose ...............................................................6.0g

Papaic digest of soybean meal............................3.0g

NaCl....................................................................2.5g

Sodium thioglycolate..........................................1.8g

Sodium formaldehyde sulfoxylate......................1.0g

L-Cystine ...........................................................0.25g

NaN3 .................................................................0.15g

Neomycin sulfate..............................................0.15g

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–118°C. Pour into sterile

Petri dishes or leave in tubes.

Caution: Sodium azide is toxic. Azides also react

with metals and disposal must be highly diluted.

Use: For the selective isolation of pathogenic

Clostridium species from specimens containing

mixed flora.