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Coagulase Mannitol Agar

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................14.5g

Pancreatic digest of casein................................10.5g

D-Mannitol ........................................................10.0g

Brain heart infusion ............................................5.0g

NaCl....................................................................3.5g

Papaic digest of soybean meal............................3.5g

Bromcresol Purple ............................................0.02g

Rabbit plasma with 0.15% EDTA............... 100.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept rabbit plasma, to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat

while stirring until boiling. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Add rabbit plasma with

0.15% EDTA to a final concentration of 7–15%. Mix

thoroughly. Pour into sterile Petri dishes in 18.0mL

volume per plate.

Use: For the cultivation and differentiation of Sta

phylococcus aureus from other Staphylococcus spe

cies based on coagulase production and mannitol fer

mentation.