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Coletsos Medium

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

Coletsos Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1625mL:

Potato starch......................................................10.0g

Gelatin.................................................................4.0g

Asparagine ........................................................2.25g

KH2PO4...............................................................1.5g

Na-glutamate.......................................................1.0g

Na-pyruvate ........................................................1.0g

Mg-citrate........................................................0.375g

Litmus ...............................................................0.25g

Malachite green.................................................0.25g

MgSO4 ..............................................................0.15g

Activated carbon .................................................0.1g

Oligonucleotide mixture ..................................3.0mg

Homogenized egg ...................................... 625.0mL

Glycerol .......................................................... 7.5mL

Egg Mixture:

Composition per liter:

Whole eggs ......................................................18–24

Preparation of Egg Mixture: Use fresh eggs,

less than 1 week old. Scrub the shells with soap. Let

stand in a soap solution for 30 min. Rinse in running

water. Soak eggs in 70% ethanol for 15 min. Break

the eggs into a sterile container. Separate egg whites

from egg yolks. Combine 8 parts egg white with 2

parts egg yolk. Homogenize by shaking. Filter

through four layers of sterile cheesecloth into a ster

ile graduated cylinder. Measure out 1.0L.

Preparation of Medium: Add glycerol to

600.0mL of distilled/deionized water. Mix thorough

ly. Add remaining components, except egg mixture.

Bring volume to 1.0L. Mix thoroughly. Gently heat

while stirring and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°C. Asepti

cally add 625.0mL of egg mixture. Mix thoroughly.

Distribute into sterile screw-capped tubes. Place

tubes in a slanted position. Inspissate at 85°C (moist

heat) for 45 min.

Use: For the cultivation of Mycobacterium tuberculo

sis.